4LOR
C1s CUB1-EGF-CUB2 in complex with a collagen-like peptide from C1q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.353, 71.173, 98.393 |
| Unit cell angles | 90.00, 111.12, 90.00 |
Refinement procedure
| Resolution | 48.570 - 2.500 |
| R-factor | 0.1986 |
| Rwork | 0.196 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.741 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.570 | 2.690 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 14918 | |
| Completeness [%] | 93.2 | 86.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 100 mM NaBr, 24% PEG 3350 , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
