4LOO
Structural basis of autoactivation of p38 alpha induced by TAB1 (Monoclinic crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.340, 73.580, 59.190 |
| Unit cell angles | 90.00, 91.15, 90.00 |
Refinement procedure
| Resolution | 46.110 - 1.950 |
| R-factor | 0.20033 |
| Rwork | 0.198 |
| R-free | 0.25024 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3que |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.596 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.180 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.061 | 0.758 |
| Number of reflections | 27019 | |
| <I/σ(I)> | 12.3 | 2 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | 25% medium-molecular weight PEG Smears, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
