4LO7
HA70(D3)-HA17-HA33
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 159.590, 187.490, 120.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.202 - 3.730 |
| R-factor | 0.2691 |
| Rwork | 0.268 |
| R-free | 0.29320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.127 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.900 |
| High resolution limit [Å] | 3.730 | 3.730 |
| Rmerge | 0.119 | 0.656 |
| Number of reflections | 38152 | |
| <I/σ(I)> | 7.4 | 2.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.2 | 292 | 0.1 M Tris, 0.1 M sodium chloride, 6% PEG2000, pH 8.2, EVAPORATION, temperature 292.0K |
