4LO5
HA70-alpha2,3-SiaLC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97952 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 260.740, 260.740, 260.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.717 - 2.700 |
| R-factor | 0.1906 |
| Rwork | 0.190 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.797 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.600 | 2.900 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.075 | 0.636 |
| Number of reflections | 41439 | |
| <I/σ(I)> | 12 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.4 | 292 | 0.1 M sodium acetate, 1.5 M ammonium chloride, pH 4.4, EVAPORATION, temperature 292.0K |
