4LNH

Crystal structure of uridine phosphorylase from Vibrio fischeri ES114, NYSGRC Target 29520.

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	H 3 2
Unit cell lengths	164.732, 164.732, 58.168
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.130 - 2.300
R-factor	0.1719
Rwork	0.170
R-free	0.21240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qpb
RMSD bond length	0.009
RMSD bond angle	1.331
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.073	0.056	0.713
Number of reflections	13419
<I/σ(I)>	12.7
Completeness [%]	99.4	95.5	100
Redundancy	11	10.1	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.2 M ammonium sulfate, 0.1 M HEPES:NaOH, pH 8.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K