4LMW
Crystal structure of glutathione transferase GSTFuA3 from Phanerochaete chrysosporium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-27 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97911 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 87.000, 87.000, 225.703 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.164 - 2.099 |
R-factor | 0.1883 |
Rwork | 0.187 |
R-free | 0.21590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g19 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.085 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.164 | 2.210 |
High resolution limit [Å] | 2.099 | 2.100 |
Rmerge | 0.064 | 0.490 |
Number of reflections | 30431 | |
<I/σ(I)> | 25.1 | 4 |
Completeness [%] | 99.6 | 97.4 |
Redundancy | 14.5 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch under oil | 4.6 | 293 | 0.1M Sodium acetate, 2M Sodium formate, pH 4.6, microbatch under oil, temperature 293K |