4LMT
Structure of The N-terminal domain of Coronavirus Nucleocapsid Protein complexed with NSC663284
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 65 |
Unit cell lengths | 81.911, 81.911, 42.873 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.732 - 1.710 |
R-factor | 0.2402 |
Rwork | 0.236 |
R-free | 0.27860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j3k |
RMSD bond length | 0.008 |
RMSD bond angle | 1.373 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.770 |
High resolution limit [Å] | 1.710 | 1.710 |
Number of reflections | 17432 | |
Completeness [%] | 97.5 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% PEG1500, 0.25M SPG, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |