4LML
GLIC double mutant I9'A T25'A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-13 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 122.803, 127.997, 190.343 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.932 - 3.800 |
R-factor | 0.2509 |
Rwork | 0.246 |
R-free | 0.30170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ehz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.222 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.120 | |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.230 | 1.239 |
Number of reflections | 24202 | |
<I/σ(I)> | 12 | 1.9 |
Completeness [%] | 97.2 | 92 |
Redundancy | 14 | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 10-12% PEG4000, 225 mM ammonium sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |