4LMG
Crystal structure of AFT2 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.28162 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.536, 127.453, 70.766 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.200 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.23600 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.682 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.931 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.078 | 0.559 |
| Number of reflections | 36252 | |
| <I/σ(I)> | 16.5 | 3.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 23% PEG2000 MME, 0.2 M MAGNESIUM CHLORIDE, 0.1 M BIS-TRIS, PH 6.6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
