4LM8
Crystal structure of the outer membrane decaheme cytochrome MtrC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.120, 90.440, 154.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.804 - 1.800 |
| R-factor | 0.1676 |
| Rwork | 0.166 |
| R-free | 0.20320 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.189 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | phenix/autosol |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.700 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.087 | 0.388 |
| Number of reflections | 67767 | |
| <I/σ(I)> | 12 | 3.4 |
| Completeness [%] | 100.0 | 97.6 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 289 | 21% PEG 6000, 0.1 M CaCl2, 0.2 M Sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
