4LLN
Crystal structure of S. aureus MepR-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.343, 76.231, 109.410 |
| Unit cell angles | 90.61, 104.75, 106.27 |
Refinement procedure
| Resolution | 44.835 - 2.842 |
| R-factor | 0.2076 |
| Rwork | 0.206 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z9c |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.915 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX ((Phaser)) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.840 |
| Rmerge | 0.096 |
| <I/σ(I)> | 31.73 |
| Completeness [%] | 91.0 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.14M CaCl2, 0.7M Na Acetate, 14% 2-propanol, 30% glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
