4LLF

Crystal structure of Cucumber Necrosis Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	295
Detector technology	CCD
Collection date	2013-01-15
Detector	BRUKER SMART 6000
Wavelength(s)	1.5418
Spacegroup name	I 2 3
Unit cell lengths	384.000, 384.000, 384.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.309 - 2.889
R-factor	0.2142
Rwork	0.213
R-free	0.24180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2tbv
RMSD bond length	0.011
RMSD bond angle	1.491
Data reduction software	SAINT
Data scaling software	SHELX
Phasing software	PHENIX (1.8.2_1309)
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	75.309
High resolution limit [Å]	2.889
Number of reflections	134159
Completeness [%]	64.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	Virus concentration was adjusted to 10 mg/ml in 20 mM sodium acetate buffer at pH 5.0. The reservoir contained 0.16 M PIPES buffer and 3.2 M sodium formate, pH 6.0. Drop composed of 10 microliters reservoir and virus solutions., VAPOR DIFFUSION, HANGING DROP, temperature 295K