4LLB

Crystal Structure of MOZ double PHD finger histone H3K14ac complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-03
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91732
Spacegroup name	C 1 2 1
Unit cell lengths	138.630, 32.230, 74.730
Unit cell angles	90.00, 94.44, 90.00

Refinement procedure

Resolution	29.100 - 2.500
R-factor	0.2222
Rwork	0.219
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ljn
RMSD bond length	0.005
RMSD bond angle	0.903
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.100	29.100	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge		0.104	0.317
Total number of observations		4306	6833
Number of reflections	11753
<I/σ(I)>	12.4	4.7	2.1
Completeness [%]	99.6	98.2	98.3
Redundancy	9.5	10.6	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	283	100 mM Tris-Cl, 200 mM Li2SO4, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, temperature 283K