4LLB
Crystal Structure of MOZ double PHD finger histone H3K14ac complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91732 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.630, 32.230, 74.730 |
| Unit cell angles | 90.00, 94.44, 90.00 |
Refinement procedure
| Resolution | 29.100 - 2.500 |
| R-factor | 0.2222 |
| Rwork | 0.219 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ljn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.903 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.100 | 29.100 | 2.640 |
| High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
| Rmerge | 0.104 | 0.317 | |
| Total number of observations | 4306 | 6833 | |
| Number of reflections | 11753 | ||
| <I/σ(I)> | 12.4 | 4.7 | 2.1 |
| Completeness [%] | 99.6 | 98.2 | 98.3 |
| Redundancy | 9.5 | 10.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 283 | 100 mM Tris-Cl, 200 mM Li2SO4, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, temperature 283K |
