4LKU

Structure of the C-terminal domain of the E. coli mechanosensitive channel of large conductance

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-01-09
Detector	PSI PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	32.250, 55.120, 32.850
Unit cell angles	90.00, 93.58, 90.00

Refinement procedure

Resolution	23.721 - 1.450
R-factor	0.1717
Rwork	0.169
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.022
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.786	23.721	1.530
High resolution limit [Å]	1.450	4.590	1.450
Rmerge		0.095	0.742
Total number of observations		4336	16445
Number of reflections	19178
<I/σ(I)>	6.2	4.1	0.9
Completeness [%]	94.2	96.2	93.5
Redundancy	5.9	6.7	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	0.1M sodium acetate pH 4.6, 0.34M ammonium acetate, 34% PEG 2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 277K