4LJQ
Crystal structure of the catalytic core of E3 ligase HOIP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2012-05-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.209, 47.767, 111.145 |
| Unit cell angles | 101.39, 90.12, 98.92 |
Refinement procedure
| Resolution | 29.600 - 2.450 |
| R-factor | 0.2088 |
| Rwork | 0.206 |
| R-free | 0.24270 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.790 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0046) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.600 | 30.000 | 2.590 |
| High resolution limit [Å] | 2.450 | 7.140 | 2.450 |
| Rmerge | 0.108 | 0.053 | 0.473 |
| Number of reflections | 30946 | 2464 | 7499 |
| <I/σ(I)> | 10.07 | 21.43 | 2.64 |
| Completeness [%] | 91.7 | 94.1 | 71 |
| Redundancy | 3.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 20% PEG 12000, 800 mM LiCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
