4LJL

Structural insights into the unique single-stranded DNA binding mode of DNA processing protein A from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	171.090, 107.860, 37.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.760 - 2.200
R-factor	0.1718
Rwork	0.170
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ljk
RMSD bond length	0.006
RMSD bond angle	0.976
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.620
High resolution limit [Å]	2.200
Number of reflections	34651
Completeness [%]	95.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	25% PEG3350, 100 mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K