4LJK
Structural insights into the unique single-stranded DNA binding mode of DNA processing protein A from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.880, 42.430, 181.330 |
| Unit cell angles | 90.00, 108.65, 90.00 |
Refinement procedure
| Resolution | 59.227 - 2.354 |
| R-factor | 0.1897 |
| Rwork | 0.186 |
| R-free | 0.25370 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.058 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 59.510 |
| High resolution limit [Å] | 2.350 |
| Number of reflections | 70980 |
| Completeness [%] | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 25% PEG3350, 100 mM HEPES, 100 mM potassium thiocyanate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
