4LJ6
ClpB NBD2 from T. thermophilus in complex with AMPPCP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2006-04-07 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97926 |
Spacegroup name | P 65 |
Unit cell lengths | 74.050, 74.050, 118.670 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.900 |
R-factor | 0.21923 |
Rwork | 0.217 |
R-free | 0.26236 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lj5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.227 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.074 | 0.554 |
Number of reflections | 28959 | |
<I/σ(I)> | 14.1 | 3.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M sodium citrate, 0.1M HEPES/NaOH, 20% isopropanol, 2mM AMPPCP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |