4LIV
Structure of YcfD, a Ribosomal oxygenase from Escherichia coli in complex with Cobalt and succinic acid.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-21 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.010, 75.010, 209.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.040 - 2.700 |
| R-factor | 0.1979 |
| Rwork | 0.195 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4liu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.911 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 70.601 | 60.937 | 2.850 |
| High resolution limit [Å] | 2.700 | 8.540 | 2.700 |
| Rmerge | 0.034 | 0.895 | |
| Total number of observations | 3567 | 16014 | |
| Number of reflections | 16951 | ||
| <I/σ(I)> | 10.7 | 20.5 | 0.9 |
| Completeness [%] | 99.0 | 96.1 | 99.8 |
| Redundancy | 6.6 | 5.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 285 | 10mM Cobalt chloride, 50mM succinic acid pH 4.2, 1.2M 1,6 hexanediol., VAPOR DIFFUSION, HANGING DROP, temperature 285K |
