4LIV

Structure of YcfD, a Ribosomal oxygenase from Escherichia coli in complex with Cobalt and succinic acid.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-09-21
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	75.010, 75.010, 209.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.040 - 2.700
R-factor	0.1979
Rwork	0.195
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4liu
RMSD bond length	0.005
RMSD bond angle	0.911
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.601	60.937	2.850
High resolution limit [Å]	2.700	8.540	2.700
Rmerge		0.034	0.895
Total number of observations		3567	16014
Number of reflections	16951
<I/σ(I)>	10.7	20.5	0.9
Completeness [%]	99.0	96.1	99.8
Redundancy	6.6	5.9	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	285	10mM Cobalt chloride, 50mM succinic acid pH 4.2, 1.2M 1,6 hexanediol., VAPOR DIFFUSION, HANGING DROP, temperature 285K