4LI4

Crystal structure of HCoV-OC43 N-NTD complexed with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	81.919, 81.919, 42.893
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.814 - 1.710
R-factor	0.2499
Rwork	0.249
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j3k
RMSD bond length	0.018
RMSD bond angle	2.228
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.710	1.710
Rmerge	0.030
Number of reflections	17535
Completeness [%]		97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2M SPG, 25% PEG1500, 5mM AMP, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K