4LH2
Structure of mouse 1-Pyrroline-5-Carboxylate Dehydrogenase (ALDH4A1) complexed with succinate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 148 |
Detector technology | IMAGE PLATE |
Collection date | 2012-08-24 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.884, 94.091, 132.339 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.677 - 1.673 |
R-factor | 0.1649 |
Rwork | 0.164 |
R-free | 0.18880 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4v9j |
RMSD bond length | 0.006 |
RMSD bond angle | 1.032 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.677 | 19.677 | 1.760 |
High resolution limit [Å] | 1.673 | 5.290 | 1.670 |
Rmerge | 0.032 | 0.342 | |
Total number of observations | 20341 | 54933 | |
Number of reflections | 118798 | ||
<I/σ(I)> | 16.3 | 16.7 | 2.2 |
Completeness [%] | 97.0 | 98 | 87.9 |
Redundancy | 4.6 | 5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 15-25% PEG 3350, 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |