4LGH
Crystal structure of 1NM-PP1 bound to analog-sensitive Src kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-07-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.264, 62.654, 74.325 |
| Unit cell angles | 100.38, 91.01, 90.07 |
Refinement procedure
| Resolution | 32.930 - 2.840 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yoj |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.798 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.3) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 2.950 |
| High resolution limit [Å] | 2.840 | 6.130 | 2.840 |
| Rmerge | 0.149 | 0.133 | 0.291 |
| Number of reflections | 16756 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 95.5 | 99.8 | 80.2 |
| Redundancy | 3.4 | 3.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100 mM MES, 50 mM NaAc, 12% glycerol, 6% PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
