4LG6
Crystal structure of ANKRA2-CCDC8 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-09 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.726, 49.791, 41.243 |
| Unit cell angles | 90.00, 98.23, 90.00 |
Refinement procedure
| Resolution | 26.149 - 1.800 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.20850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3so8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.352 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.818 | 26.135 | 1.900 |
| High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
| Rmerge | 0.020 | 0.650 | |
| Total number of observations | 1688 | 7328 | |
| Number of reflections | 14082 | ||
| <I/σ(I)> | 16.2 | 24.8 | 1.2 |
| Completeness [%] | 97.0 | 98.5 | 94.8 |
| Redundancy | 3.7 | 3.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 25% PEG3350, 0.2 M sodium chloride, 0.1 M HEPES, pH 7.5, vapor diffusion, temperature 291K |
