4LFT
Structure of alpha-elapitoxin-Dpp2d isolated from Black Mamba (Dendroaspis polylepis) venom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-24 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.595, 38.270, 39.419 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.460 - 1.700 |
| R-factor | 0.19916 |
| Rwork | 0.198 |
| R-free | 0.22371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ntn |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.267 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.460 | 1.744 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.045 | 0.483 |
| Number of reflections | 22047 | |
| <I/σ(I)> | 27.2 | 1.9 |
| Completeness [%] | 92.8 | 74.79 |
| Redundancy | 5.53 | 2.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | protein was dissolved in 2mM HCl, 5 mg/mL. reservoir: 0.1M TRIS-HCL, 2% (v/v) dioxane, 65% (v/v) 2-methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
