4LFN

Crystal Structure of D-galactose-6-phosphate isomerase in complex with D-ribulose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97932
Spacegroup name	P 21 21 2
Unit cell lengths	108.261, 116.009, 54.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.650
Rwork	0.241
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lfk
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.650	4.480	1.650
Rmerge	0.102	0.069	0.597
Number of reflections	82263
<I/σ(I)>	8.2
Completeness [%]	97.9	88.6	95.9
Redundancy	12.3	11.7	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 0.2M potassium formate, VAPOR DIFFUSION, HANGING DROP, temperature 295K