4LEY
Structure of mouse cGAS bound to 18 bp DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 153 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-03-31 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.377, 145.895, 100.963 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 40.773 - 2.500 |
| Rwork | 0.161 |
| R-free | 0.19110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | human cGAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.942 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.107 | 0.716 |
| <I/σ(I)> | 18.3 | 2 |
| Completeness [%] | 99.0 | 1 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 24-36% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
