4LEY
Structure of mouse cGAS bound to 18 bp DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 153 |
Detector technology | IMAGE PLATE |
Collection date | 2013-03-31 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.542 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.377, 145.895, 100.963 |
Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
Resolution | 40.773 - 2.500 |
Rwork | 0.161 |
R-free | 0.19110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | human cGAS |
RMSD bond length | 0.005 |
RMSD bond angle | 0.942 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.800 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.107 | 0.716 |
<I/σ(I)> | 18.3 | 2 |
Completeness [%] | 99.0 | 1 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 24-36% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |