4LE9
Crystal structure of a chimeric c-Src-SH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 68.239, 68.239, 46.495 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.699 - 1.344 |
| R-factor | 0.1372 |
| Rwork | 0.136 |
| R-free | 0.16380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hvu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.155 |
| Data scaling software | SCALA (0.1.30) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.097 | 1.370 |
| High resolution limit [Å] | 1.344 | 1.340 |
| Rmerge | 0.025 | 0.542 |
| Number of reflections | 14621 | |
| <I/σ(I)> | 29.5 | 3 |
| Completeness [%] | 99.4 | 98 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 2.2 M ammonium sulphate, 10% Glicerol, 5% PEG 300, 0.01 M non-detergent sulphobetaine 201,0.1M Sodium Acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
