4LDU
Crystal structure of the DNA binding domain of Arabidopsis thaliana auxin response factor 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.93340 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.613, 91.502, 77.182 |
| Unit cell angles | 90.00, 114.28, 90.00 |
Refinement procedure
| Resolution | 38.380 - 2.150 |
| R-factor | 0.19771 |
| Rwork | 0.195 |
| R-free | 0.25206 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.626 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.063 | 0.430 |
| Number of reflections | 23983 | |
| <I/σ(I)> | 11 | 2.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 nL 13 mg/mL ARF5DBD +100 nl crystallization buffer (20 mM TRIS 7.5, 12.5% PEG MME 2000), VAPOR DIFFUSION, SITTING DROP, temperature 293K |
