4LDF
Crystal Structure of CpBRD2 from cryptosporidium, cgd3_3190
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.892, 44.642, 59.537 |
| Unit cell angles | 90.00, 96.50, 90.00 |
Refinement procedure
| Resolution | 33.530 - 2.000 |
| R-factor | 0.1864 |
| Rwork | 0.184 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.1) |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.118 | 0.052 | 0.756 |
| Number of reflections | 13557 | ||
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 99.9 | 99 | 99.4 |
| Redundancy | 3.4 | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 30 % PEG 2K MME 0.1 M Potassium thiocyanate 15% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
