4LD1

Structural analysis of the microcephaly protein CPAP G-box domain suggests a role in centriole elongation.

Experimental procedure

Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	41.632, 50.254, 60.345
Unit cell angles	90.00, 108.56, 90.00

Refinement procedure

Resolution	39.467 - 1.440
R-factor	0.1549
Rwork	0.154
R-free	0.17970
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.204
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (AUTOSOL)
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.470
High resolution limit [Å]	1.440
Number of reflections	42577
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.1 M MES/Imidazole, 0.03 M sodium nitrate, 0.03 M disodium hydrogen phosphate, 0.03 M ammonium sulfate, 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD , VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 6.5