4LCX

The structure of hemagglutinin from avian-origin H7N9 influenza virus (A/Shanghai/1/2013)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	74.880, 225.982, 75.336
Unit cell angles	90.00, 117.92, 90.00

Refinement procedure

Resolution	49.882 - 3.094
R-factor	0.2283
Rwork	0.226
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.343
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.094	3.100
Number of reflections	39468
Completeness [%]	99.6	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	18% PEG 8000, 18% Ethylene glycol, 0.12 M Di-Ethyleneglycol, 0.12 M Tri-Ethyleneglycol, 0.12 M TetraEthyleneglycol, 0.12 M Penta-Ethyleneglycol, 0.1 M Tris, 0.1 M Bicine, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K