4LCJ
CtBP2 in complex with substrate MTOB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.151, 140.605, 135.126 |
| Unit cell angles | 90.00, 97.87, 90.00 |
Refinement procedure
| Resolution | 32.303 - 2.860 |
| R-factor | 0.2158 |
| Rwork | 0.214 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.219 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.960 |
| High resolution limit [Å] | 2.860 | 6.160 | 2.860 |
| Rmerge | 0.093 | 0.052 | 0.389 |
| Number of reflections | 73374 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 99.4 | 98.6 | 98.8 |
| Redundancy | 4.1 | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 200 mM potassium nitrate, 15-20% PEG 3350, 100 mM bis tris propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
