4LCB
Structure of Vps4 homolog from Acidianus hospitalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 |
| Unit cell lengths | 95.962, 95.962, 79.499 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.080 |
| R-factor | 0.2156 |
| Rwork | 0.213 |
| R-free | 0.25430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lgm |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.442 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.083 | 0.055 | 0.673 |
| Number of reflections | 24654 | ||
| <I/σ(I)> | 17.2 | ||
| Completeness [%] | 99.1 | 91.2 | 100 |
| Redundancy | 9.3 | 8.3 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M MgCl2, 0.1 M HEPES pH 7.5, 30 % PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
