4LB6
Crystal structure of PKZ Zalpha in complex with ds(CG)6 (tetragonal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 70 |
| Detector technology | PIXEL |
| Collection date | 2012-09-29 |
| Detector | PILATUS 6M-F |
| Wavelength(s) | 0.97 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 110.910, 110.910, 43.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.072 - 1.800 |
| R-factor | 0.1841 |
| Rwork | 0.181 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lb5 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.062 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.550 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | 0.679 |
| Number of reflections | 12161 | |
| <I/σ(I)> | 11.3 | 2.2 |
| Completeness [%] | 96.4 | 96 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 2.0 M ammonium sulfate, 0.1 M sodium acetate, 7.5 % glycerol, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
