4LAD

Crystal Structure of the Ube2g2:RING-G2BR complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	58.246, 58.246, 158.431
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.123 - 2.300
R-factor	0.2302
Rwork	0.226
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h8k
RMSD bond length	0.009
RMSD bond angle	1.220
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.044	0.027	0.396
Number of reflections	12528
<I/σ(I)>	18.4	54.2	2.6
Completeness [%]	96.9	96	90.3
Redundancy	8.2	8.4	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	25% PEG-3350, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K