4LAC

Crystal Structure of Protein Phosphatase 2A (PP2A) and PP2A phosphatase activator (PTPA) complex with ATPgammaS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	57.070, 100.186, 167.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.820
R-factor	0.19057
Rwork	0.188
R-free	0.24226
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3C5W PDB ENTRY 2IE3 PDB ENTRY 2HV6
RMSD bond length	0.006
RMSD bond angle	1.165
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (MR)
Refinement software	REFMAC (5.6.0119)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.820
Number of reflections	23656
<I/σ(I)>	11.1	2.02
Completeness [%]	99.1	90.4
Redundancy	7.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1M MES at pH6.5 and 10-12% PEG20,000, 1mM ATPgammaS, VAPOR DIFFUSION, SITTING DROP, temperature 298K