4L9U
Structure of C-terminal coiled coil of RasGRP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 24.878, 165.047, 28.319 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.911 - 1.601 |
R-factor | 0.2024 |
Rwork | 0.201 |
R-free | 0.23510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.146 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.911 | 27.910 | 1.630 |
High resolution limit [Å] | 1.600 | 8.470 | 1.600 |
Number of reflections | 16098 | ||
Completeness [%] | 98.9 | 89.7 | 97.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.6 | 296 | 20 mM sodium acetate, 22% PEG 3350, 100 mM lithium sulfate, 0.4% formamide, pH 3.6, VAPOR DIFFUSION, SITTING DROP, temperature 296K |