4L9T
Crystal structure of MepR F27L mutant from multidrug resistant S. aureus clinical isolate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.441, 113.122, 49.936 |
Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
Resolution | 24.720 - 1.794 |
R-factor | 0.1717 |
Rwork | 0.170 |
R-free | 0.20340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.894 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((Phaser)) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 1.790 |
Number of reflections | 32048 |
Completeness [%] | 96.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M (NH4)2HPO4, 20% PEG 3350, pH 8, vapor diffusion, hanging drop, temperature 293K |