4L9J

Crystal structure of S. aureus MepR in DNA-binding conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97236
Spacegroup name	P 41 21 2
Unit cell lengths	98.806, 98.806, 151.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.040 - 3.450
R-factor	0.2288
Rwork	0.225
R-free	0.26430
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.649
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((Phaser))
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.450
Number of reflections	10448
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	2 mM CuCl2, 50 mM Tris HCl pH 8.5, 1.8 M Li2SO4, and 0.5 mM spermine, VAPOR DIFFUSION, HANGING DROP, temperature 293K