4L8G
Crystal Structure of K-Ras G12C, GDP-bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9999 |
Spacegroup name | P 63 |
Unit cell lengths | 82.146, 82.146, 39.513 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.731 - 1.521 |
R-factor | 0.151 |
Rwork | 0.148 |
R-free | 0.18040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gft |
RMSD bond length | 0.019 |
RMSD bond angle | 1.868 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.2.4) |
Refinement software | PHENIX (dev_1402) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.550 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.042 | 0.349 |
Number of reflections | 23541 | |
<I/σ(I)> | 38.873 | 5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.7 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 21% PEG3350, 0.2M CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |