4L83
Structure of a putative Ubiquitin-conjugating enzyme E2 from Brugia malayi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 34.850, 52.030, 108.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.970 - 1.700 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2grn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.338 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.2) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.740 | |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.056 | 0.040 | 0.424 |
Number of reflections | 22536 | 308 | 1665 |
<I/σ(I)> | 19.48 | 35.67 | 3.92 |
Completeness [%] | 99.9 | 97.5 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 28mg/ml BrmaA.00250.a, 200mM sodium malonate dibasic, 20% PEG3350, 20% ethylene glycol for cryoprotection. , pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |