4L69
Rv2372c of Mycobacterium tuberculosis is RsmE like methyltransferse
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 81.940, 81.940, 115.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.750 - 2.900 |
| R-factor | 0.2009 |
| Rwork | 0.199 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vhy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.090 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.940 | |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.067 | |
| Number of reflections | 8836 | |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 297 | pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
