4L5S

p202 HIN1 in complex with 12-mer dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-31
Detector	ADSC QUANTUM 4
Wavelength(s)	1.075
Spacegroup name	C 2 2 21
Unit cell lengths	111.309, 156.118, 117.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.970 - 2.940
R-factor	0.207
Rwork	0.204
R-free	0.25500
Starting model (for MR)	4l5q
RMSD bond length	0.008
RMSD bond angle	1.491
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.970
High resolution limit [Å]	2.943
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	2.2 M ammonium sulfate, 0.1 M sodium cacodylate pH 5.2-5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K