4L5I
Crystal structures of the LsrR proteins complexed with phospho-AI-2 and its two different analogs reveal distinct mechanisms for ligand recognition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97940 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.744, 80.535, 103.635 |
| Unit cell angles | 90.00, 112.18, 90.00 |
Refinement procedure
| Resolution | 47.980 - 3.210 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.980 | 3.310 |
| High resolution limit [Å] | 3.210 | 3.200 |
| Number of reflections | 19846 | |
| Completeness [%] | 79.1 | 90.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M HEPES sodium, 17% isopropanol, 2% PEG3350, 2% Tacsimate, pH 7.0, VAPOR DIFFUSION, temperature 293K |
