4L4S
Structural characterisation of the NADH binary complex of human lactate dehydrogenase M isozyme
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9173 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 84.317, 84.317, 276.691 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.191 - 2.900 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.24630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 84.317 | 29.191 | 3.060 |
| High resolution limit [Å] | 2.900 | 9.170 | 2.900 |
| Rmerge | 0.052 | 0.771 | |
| Total number of observations | 4520 | 18405 | |
| Number of reflections | 23067 | ||
| <I/σ(I)> | 7.7 | 12.3 | 1 |
| Completeness [%] | 99.8 | 97.1 | 99.9 |
| Redundancy | 5.7 | 5.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 10 % PEG 8000, 100 mM HEPES, 20 % ethylene glycol, 10 % acetonitrile, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
