4L45
Crystal structures of human p70S6K1-T389E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-07 |
| Detector | CCD ADSC unsupported-q315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.191, 81.191, 110.744 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.540 - 2.900 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.28650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a62 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.092 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.900 | 6.030 |
| Rmerge | 0.077 | 0.037 |
| Number of reflections | 9796 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 5.3 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.5 | 289 | 1.4M sodium citrate, 0.1M sodium cacodylate, pH 6.5, hanging drop, temperature 289K |
