4L3N

Crystal structure of the receptor-binding domain from newly emerged Middle East respiratory syndrome coronavirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	45.361, 108.065, 124.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.552 - 2.130
R-factor	0.14707
Rwork	0.144
R-free	0.20602
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.335
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.552
High resolution limit [Å]	2.130	2.130
Number of reflections	34770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	27% v/v PEG3350, 0.2 M magnesium chloride, 0.1 M Bis-Tris, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K