4L3J

Crystal structures of human p70S6K1 kinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-20
Detector	CCD ADSC unsupported-q315
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	70.511, 70.511, 150.484
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.2125
Rwork	0.210
R-free	0.25460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a62
RMSD bond length	0.016
RMSD bond angle	1.333
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	22617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1