4L3I

Structure of the microtubule associated protein PRC1 (Protein Regulator of Cytokinesis 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9787
Spacegroup name	P 1 21 1
Unit cell lengths	61.205, 210.782, 112.294
Unit cell angles	90.00, 105.81, 90.00

Refinement procedure

Resolution	29.734 - 3.600
R-factor	0.2617
Rwork	0.257
R-free	0.29990
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.762
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol ((phenix))
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.680
High resolution limit [Å]	3.550	3.550
Number of reflections	31794
Completeness [%]	96.2	83.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	Concentration: 5-10 mg/ml Protein buffer: 80 mM PIPES (pH 6.8), 1 mM MgCl2, 1 mM EGTA, 150 mM KCl, VAPOR DIFFUSION, HANGING DROP, temperature 294K