4L26
Crystal structure of DNA duplex containing consecutive T-T mispairs (Br-derivative)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 277 |
| Collection date | 2012-10-22 |
| Wavelength(s) | 1.0, 0.91942, 0.92006 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.360, 39.310, 30.460 |
| Unit cell angles | 90.00, 99.41, 90.00 |
Refinement procedure
| Resolution | 21.940 - 1.400 |
| Rwork | 0.232 |
| R-free | 0.26620 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.964 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHENIX |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 21.940 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 12212 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | Sodium Cacodylate, Spermine tetrahydrochloride, 2-methyl-2,4-pentanediol, ammonium chloride , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
